Published erratum appears in Nature 1992 Dec 3;360(6403):491

Abrogation by c-myc of G1 phase arrest induced by RB protein but not by p53.

"c-myc" "eliminates" "G1 phase arrest"
"G1" "is a phase of" "cell cycle"
"RB protein" "is a synonym for" "retinoblastoma protein"
"G1 phase arrest" "induced by" "RB protein"
"G1 phase arrest" "not induced by" "p53 protein"

Goodrich DW,
Lee WH

"this" "authored by" "Goodrich DW"
"this" "authored by" "Lee WH"

"this" "authored at" "Center for Molecular Medicine"
"this" "authored at" "University of Texas Health Science Center"
"this" "authored at" "San Antonio 78245"

Inactivating mutations of the retinoblastoma gene (RB) are found in a wide variety of tumour cells.

"RB protein" "when mutated can be" "inactivated"
"RB protein" "when mutated is" "RB protein mutated"
"RB protein mutated" "is found in" "tumor cells"

Replacement of wild-type RB can suppress the tumorigenicity of some of these cells, suggesting that the RB protein (Rb) may negatively regulate cell growth.

"RB protein mutated" "when replaced by suppresses tumorigenicity" "RB protein"
"RB protein" "negatively regulates" "cell growth"

As activation of c-myc expression promotes cell proliferation and blocks differentiation,
it may positively regulate cell growth.

"cell growth" " is promoted by expression of" "c-myc protein"
"cell differentiation" " is blocked by expression of" "c-myc protein"

The c-myc protein is localized in the nucleus and can physically associate with RB protein in vitro, hence c-myc may functionally antagonize RB function.

"c-myc protein" "is located in" "the cell nucleus"
"c-myc protein" "associates with in vitro" "RB protein"
"c-myc protein" "may antagonize function of" "RB protein"

Microinjection of Rb in G1 phase reversibly arrests cell-cycle progression.

"RB protein" "reversibly arrests progression of" "cell cycle"
"RB protein" "can be microinjected to arrest" "cell cycle"

Here we co-inject RB protein with c-myc, EJ-ras, c-fos or c-jun protein.
"RB protein" "can be comicroinjected with" "c-myc"

Co-injection of c-myc, but not EJ-ras, c-fos or c-jun, inhibits the ability of Rb to arrest the cell cycle.

"RB protein" "required for inhibition of" "cell-cycle "
"c-myc protein" "required for inhibition of" "cell-cycle "

"RB protein" "can be co-injected with" "c-myc protein"

The c-myc does not inhibit the activity of another tumour supressor, p53 (ref. 12).

"c-myc protein" "does not inhibit" "p53 protein"
"p53 protein" "is a" "tumor supressor"

Thus, c-myc and RB specifically antagonize one another in the cell.
"c-myc protein" "antogonizes in the cell" "RB protein"

MeSH Terms:
Animal
Cycloheximide/pharmacology
G1 Phase/physiology*
Human
Microinjections
Protein p53/physiology*
Proto-Oncogene Proteins c-fos/physiology
Proto-Oncogene Proteins c-jun/physiology
Proto-Oncogene Proteins c-jun/pharmacology
Proto-Oncogene Proteins c-myc/physiology*
Retinoblastoma Protein/physiology*
Support,
Non-U.S. Gov't Support,
U.S. Gov't, P.H.S.

Gene Symbols:
p53
c-myc

Substances:
Cycloheximide
Retinoblastoma Protein
Proto-Oncogene Proteins c-myc
Proto-Oncogene Proteins c-jun
Proto-Oncogene Proteins c-fos
Protein p53

PMID: 1436095,
UI: 93063346