Surf on Telomerase

Goal: Visualize the DNA Binding of this reverse transcriptase

LVW 3/29/00

1) check pdb structure server

• telomerase doesn't appear

2) check swiss protenomics tools

• need primary sequence

3) check entrez

• need primary sequence for telomerase

4) select variant

• look for homo sapiens

5) examine citation

• get sequence using cut and paste (FASTA format)

6) first order structure prediction

• attempt first approach structure prediction

7) attempt fails - no similar protein in the bank

• attempt first approach structure prediction

8) back up and do secondary structure prediction

• attempt secondary structure prediction

9) request multiple approach services

10) NetPhos Results

11) PROSITE motif search Results

12) Initial Secondary structure Results

13) Final Secondary structure Results via JPred2

had to trim to < 800 AA